Maisch W F, Wachsman J T
J Bacteriol. 1967 Oct;94(4):1088-92. doi: 10.1128/jb.94.4.1088-1092.1967.
A triple auxotroph of Bacillus megaterium strain KM was lysogenized with a phage suspension from B. megaterium 899a. The lysogenic and phage-sensitive derivatives of KM were found to die at the same exponential rate during thymineless incubation, despite the fact that the lysogenic strain became induced. The lysogenic strain was also induced by mitomycin C, and died at an exponential rate which was approximately twice that of the sensitive strain. With both strains, the lethality of mitomycin C was the same in the presence and absence of thymidine; thymidine was required for maximal phage production. Mitomycin C preferentially inhibited deoxyribonucleic acid (DNA) synthesis of both strains for the first 60 min. The (DNA) synthetic ability of the lysogenic strain was subsequently restored, due to phage production. Since there was no evidence that sensitive strains of KM contained other inducible elements (prophage or probacteriocins), it is concluded that both thymineless death and mitomycin C death can occur via mechanisms not involving induction.
巨大芽孢杆菌KM菌株的一个三重营养缺陷型用来自巨大芽孢杆菌899a的噬菌体悬液进行了溶源化处理。尽管溶源菌株被诱导，但在无胸腺嘧啶培养期间，KM的溶源衍生物和噬菌体敏感衍生物以相同的指数速率死亡。溶源菌株也被丝裂霉素C诱导，并以大约是敏感菌株两倍的指数速率死亡。对于这两种菌株，在有和没有胸苷的情况下，丝裂霉素C的致死率是相同的；最大噬菌体产生需要胸苷。在最初的60分钟内，丝裂霉素C优先抑制这两种菌株的脱氧核糖核酸（DNA）合成。由于噬菌体的产生，溶源菌株的（DNA）合成能力随后得以恢复。由于没有证据表明KM的敏感菌株含有其他可诱导元件（原噬菌体或原细菌素），所以得出结论，无胸腺嘧啶死亡和丝裂霉素C死亡都可以通过不涉及诱导的机制发生。
