Suitters B T, Brogger S A
Bull World Health Organ. 1967;36(5):837-45.
In a study of some of the difficulties attending the bacteriological examination of sputa in mass campaigns against tuberculosis, the authors show that culture of specimens always yields significantly more positive results than routine microscopy alone and that sputum culture is more efficient than laryngeal-swab culture. The effect and occurrence of contamination in cultures were investigated in a special trial, and it was found that even minimal contamination adversely affects mycobacterial growth and is sufficient grounds for discarding contaminated cultures. Because of the anticipated detrimental effect of transporting specimens, a subsidiary trial was made to determine whether it would be possible to inoculate cultures in the field for subsequent examination in the laboratory; despite the high contamination rates to be expected from cultures prepared in the field, the technique might prove useful for supplementing microscopic examination in rural centres, since the positivity rate among uncontaminated cultures is not affected. It is also shown that nearly as much information may be obtained from three cultures prepared from each of two sputum specimens as from a third specimen, and at much less cost and effort.
在一项针对大规模结核病防治运动中痰液细菌学检查所面临困难的研究中，作者表明，标本培养所获得的阳性结果总是显著多于单纯的常规显微镜检查，并且痰培养比喉拭子培养更有效。在一项特别试验中对培养物中的污染影响和发生率进行了研究，结果发现即使是极少的污染也会对分枝杆菌生长产生不利影响，并且足以成为丢弃受污染培养物的理由。由于预计运输标本会产生有害影响，因此进行了一项辅助试验，以确定是否有可能在现场接种培养物以便随后在实验室进行检查；尽管预计现场制备的培养物污染率很高，但该技术可能对农村中心补充显微镜检查有用，因为未受污染培养物中的阳性率不受影响。研究还表明，从两份痰液标本中的每一份制备的三份培养物所获得的信息几乎与从第三份标本中获得的信息一样多，而且成本和工作量要少得多。
