瘤胃原生动物中含氨基乙基膦酸酯的脂质。
The aminoethylphosphonate-containing lipids of rumen protozoa.
作者信息
Dawson R M, Kemp P
出版信息
Biochem J. 1967 Nov;105(2):837-42. doi: 10.1042/bj1050837.
Abstract
- A method is presented for identifying and estimating the aminoethylphosphonate (ciliatine)-containing phospholipids in a complex mixture. 2. Evidence was obtained that the phospholipids of a pure culture of Entodinium caudatum and a mixed rumen protozoa sample contain diglyceride ciliatine, and a plasmalogen ciliatine was detected in the latter. 3. A ninhydrin-positive sphingolipid was isolated from rumen protozoa. Although chromatographically homogeneous on silica gel it contains two components, which were provisionally identified as ceramide ciliatine and ceramide phosphorylethanolamine. 4. A detailed phospholipid analysis of E. caudatum and rumen protozoa is presented. They contain no phosphatidylserine or cardiolipin, but an unidentified phosphoglyceride containing a zwitterionic amino acid is present.
摘要
- 本文介绍了一种用于鉴定和估算复杂混合物中含氨乙基膦酸（纤毛碱）的磷脂的方法。2. 有证据表明，尾草履虫纯培养物和瘤胃原生动物混合样品中的磷脂含有甘油二酯纤毛碱，且在后一种样品中检测到了缩醛磷脂纤毛碱。3. 从瘤胃原生动物中分离出一种茚三酮阳性鞘脂。尽管在硅胶上进行色谱分析时它是均一的，但它含有两种成分，暂定为神经酰胺纤毛碱和神经酰胺磷酰乙醇胺。4. 本文对尾草履虫和瘤胃原生动物进行了详细的磷脂分析。它们不含磷脂酰丝氨酸或心磷脂，但存在一种含有两性离子氨基酸的未知磷酸甘油酯。
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