铜绿假单胞菌可育菌株中的生存能力。
Viability in fertile stocks of Pseudomonas aeruginosa.
作者信息
Herman A I, Griffin P S
出版信息
J Bacteriol. 1968 May;95(5):1758-63. doi: 10.1128/jb.95.5.1758-1763.1968.
Diluent composition, time consumed in experimental manipulations, and the presence of pyocine-like lethal agents affected the viability of sexual fertility factor-positive and fertility factor-negative auxotrophic stocks of Pseudomonas aeruginosa. In the same cultures, recovery of prototrophic revertants increased as the number of viable cells dispensed per plate was reduced. As a result, the number of revertants recovered was indirectly determined by the combined activities of the three conditions affecting viability. Possible modifications by these conditions affecting viability on the expression and interpretation of the fertility factor-positive sex factor-mediated system of genetic recombination are presented.
稀释剂成分、实验操作所耗费的时间以及类绿脓菌素致死因子的存在，均会影响铜绿假单胞菌性育性因子阳性和育性因子阴性营养缺陷型菌株的生存能力。在相同培养物中，随着每平板接种的活细胞数量减少，原养型回复突变体的回收率会增加。因此，所回收的回复突变体数量是由影响生存能力的这三个条件的综合作用间接决定的。本文还介绍了这些影响生存能力的条件可能对育性因子阳性性因子介导的基因重组系统的表达和解释产生的修饰作用。
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