复苏培养基中盐浓度对热损伤粪肠球菌的影响。
Effect of salt concentration in the recovery medium on heat-injured Streptococcus faecalis.
作者信息
Beuchat L R, Lechowich R V
出版信息
Appl Microbiol. 1968 May;16(5):772-6. doi: 10.1128/am.16.5.772-776.1968.
Abstract
Properties relating to the recovery of three heat-injured strains of Streptococcus faecalis were studied. All strains were cultured in all purpose plus Tween broth (APT) at 30 C for 24 hr before being subjected to heat in fresh APT broth. APT recovery medium containing various added amounts of NaCl, KCl, MgCl(2), or KCl and MgCl(2) was used to assess the effect of salts on the recovery of thermally injured S. faecalis. It was evident that, upon exposure to heat, S. faecalis cells became sensitive to increased salt concentrations. Analyses to determine the ribonucleic acid (RNA) content of heated cells showed a reduction of cellular RNA, but the per cent reduction was not directly proportional to the per cent reduction of the viable cells.
摘要
对三种热损伤粪肠球菌菌株的恢复特性进行了研究。所有菌株在通用加吐温肉汤（APT）中于30℃培养24小时，然后在新鲜的APT肉汤中进行加热处理。使用含有不同添加量NaCl、KCl、MgCl₂或KCl和MgCl₂的APT恢复培养基来评估盐对热损伤粪肠球菌恢复的影响。很明显，在受热时，粪肠球菌细胞对盐浓度升高变得敏感。对受热细胞的核糖核酸（RNA）含量进行测定的分析表明细胞RNA减少，但减少的百分比与活细胞减少的百分比并不直接成比例。
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