从泌乳豚鼠乳腺中分离出的高分子量含核聚腺苷酸核糖核酸含有乳蛋白信使核糖核酸序列。
High-molecular-weight nuclear polyadenylate-containing ribonucleic acid isolated from the lactating guniea-pig mammary gland contains milk-protein messenger ribonucleic acid sequences.
作者信息
Bathurst I C, Craig R K, Campbell P N
出版信息
Biochem J. 1979 Aug 1;181(2):501-4. doi: 10.1042/bj1810501.
Abstract
- Steady-state poly(A)-containing nuclear RNA was isolated from the lactating guinea-pig mammary gland and analysed by sucrose-gradient centrifugation under denaturing conditions. 2. Nucleic acid-hybridization studies demonstrated the presence of small amounts of high-molecular-weight RNA species containing milk-protein mRNA sequences sedimenting at 25S. These were not found in the post-nuclear supernatant, where milk-protein sequences sedimented only between 12S and 15S; these were also the predominant species in the nuclear fraction. 3. The results are consistent with the transcription of milk-protein genes as high-molecular-weight precursor RNA species, 3-4 times as large as the active mRNA species isolated from the post-nuclear fraction.
摘要
- 从泌乳豚鼠乳腺中分离出稳态含聚腺苷酸的核RNA，并在变性条件下通过蔗糖梯度离心进行分析。2. 核酸杂交研究表明，存在少量含有乳蛋白mRNA序列的高分子量RNA种类，其在25S处沉降。在后核上清液中未发现这些种类，在那里乳蛋白序列仅在12S至15S之间沉降；这些也是核部分中的主要种类。3. 结果与乳蛋白基因转录为高分子量前体RNA种类一致，该前体RNA种类是从后核部分分离出的活性mRNA种类的3 - 4倍。
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引用本文的文献
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Inhibition of cell-free protein synthesis by low-molecular-weight nuclear polyadenylate-containing ribonucleic acid species isolated from the lactating guinea pig.从泌乳豚鼠中分离出的含低分子量核聚腺苷酸核糖核酸种类对无细胞蛋白质合成的抑制作用。
Biochem J. 1980 Feb 15;186(2):561-70. doi: 10.1042/bj1860561.
本文引用的文献
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The ribonuclease activity of crystallized pancreatic deoxyribonuclease.结晶胰脱氧核糖核酸酶的核糖核酸酶活性
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Guinea-pig milk-protein synthesis. Isolation and characterization of messenger ribonucleic acids from lactating mammary gland and identification of caseins and pre-alpha-lactalbumin as translation products in heterologous cell-free systems.豚鼠乳蛋白合成。从泌乳乳腺中分离和鉴定信使核糖核酸，并在异源无细胞系统中鉴定酪蛋白和前α-乳白蛋白作为翻译产物。
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A precursor of globin messenger RNA.珠蛋白信使核糖核酸的前体。
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Transcription of structural and intervening sequences in the ovalbumin gene and identification of potential ovalbumin mRNA precursors.卵清蛋白基因中结构序列和间隔序列的转录以及潜在卵清蛋白mRNA前体的鉴定。
Cell. 1978 Oct;15(2):671-85. doi: 10.1016/0092-8674(78)90035-1.
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Separation and partial characterization of guinea-pig caseins.豚鼠酪蛋白的分离与部分特性鉴定
Biochem J. 1978 Aug 1;173(2):633-41. doi: 10.1042/bj1730633.
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The absence of a precursor larger than 16 S to globin mRNA.不存在大于16 S的珠蛋白mRNA前体。
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Cell. 1978 Dec;15(4):1495-509. doi: 10.1016/0092-8674(78)90072-7.
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