豚鼠乳腺的核糖核酸
The ribonucleic acids of the mammary gland of the guinea pig.
作者信息
Ashby P, Campbell P N
出版信息
Biochem J. 1975 Mar;146(3):575-83. doi: 10.1042/bj1460575.
Abstract
- 32-P-labelled polyribosome preparations were made from the mammary glands of lactating and late-pregnant guinea pigs after injection of (32-P)i into the animals. 2. The RNA of polyribosomes, ribosomal subunits and that released from polyribosomes by EDTA were analysed by zone velocity centrifugation and by polyacrylamide-gel electrophoresis. 3. RNA species which have the physical properties expected for the milk protein mRNA were detected. RNA species of a size which could code for the caseins were present in lactating but not in pre-lactating mammary-gland polyribosomes.
摘要
- 在向豚鼠注射（32-P）i后，从泌乳期和妊娠后期豚鼠的乳腺制备32-P标记的多核糖体制剂。2. 通过区带速度离心和聚丙烯酰胺凝胶电泳分析多核糖体、核糖体亚基以及通过EDTA从多核糖体释放的RNA。3. 检测到具有乳蛋白mRNA预期物理性质的RNA种类。能够编码酪蛋白大小的RNA种类存在于泌乳期乳腺的多核糖体中，而不存在于泌乳前乳腺的多核糖体中。
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本文引用的文献
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Rat liver cytoplasmic ribonucleic acids: some aspects of their localization and isolation.大鼠肝脏细胞质核糖核酸：其定位与分离的某些方面
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