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自溶对某些乳酸菌摩尔生长产率的影响

Molar growth yields of certain lactic acid bacteria as influenced by autolysis.

作者信息

Moustafa H H, Collins E B

出版信息

J Bacteriol. 1968 Jul;96(1):117-25. doi: 10.1128/jb.96.1.117-125.1968.

DOI:10.1128/jb.96.1.117-125.1968
PMID:4969603
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC252261/
Abstract

Molar growth yields determined from batch cultures of Streptococcus diacetilactis and S. faecalis were appreciably greater at the peaks of maximal growth than after continued incubation and considerable autolysis. The higher molar growth yields were about equal to those determined in a continuous culture. Autolysis during logarithmic growth was minimal. The average Y value for adenosine triphosphate (ATP), determined by using limiting concentrations of glucose, galactose, lactose, and maltose for growing S. diacetilactis and limiting concentrations of glucose for growing S. lactis, S. cremoris, and S. faecalis, was 17.0. This is close to the Y (arginine) value of 17.8 determined with S. faecalis, but 62% greater than the generally accepted value of 10.5. Data are presented indicating that the often-used Y (ATP) value of 10.5 is erroneously low.

摘要

从双乙酰乳酸链球菌和粪肠球菌的分批培养物中测定的摩尔生长产量,在最大生长峰值时明显高于继续培养并发生大量自溶后的产量。较高的摩尔生长产量约等于在连续培养中测定的产量。对数生长期的自溶作用最小。通过使用限制浓度的葡萄糖、半乳糖、乳糖和麦芽糖培养双乙酰乳酸链球菌,以及使用限制浓度的葡萄糖培养乳酸链球菌、乳脂链球菌和粪肠球菌,所测定的三磷酸腺苷(ATP)的平均Y值为17.0。这接近于用粪肠球菌测定的Y(精氨酸)值17.8,但比普遍接受的10.5的值高62%。所呈现的数据表明,经常使用的10.5的Y(ATP)值错误地偏低。