从各种分枝杆菌物种中分离和鉴定分枝菌素的方法。
Methods for the separation and identification of mycobactins from various species of mycobacteria.
作者信息
White A J, Snow G A
出版信息
Biochem J. 1968 Jul;108(4):593-7. doi: 10.1042/bj1080593.
Abstract
A single pure component was isolated from mycobactin P by countercurrent distribution; its side chain is n-cis-octadec-2-enoyl; its purity and molecular structure were confirmed by mass spectrometry of its aluminium complex. The separation of ferric and of aluminium complexes of mycobactins by thin-layer chromatography is described. Mycobacterium terrae, M. marinum and M. smegmatis produce mycobactins that differ among themselves and from mycobactins P and T. A nomenclature for the mycobactins and their derivatives is suggested.
摘要
通过逆流分配法从分枝杆菌素P中分离出单一纯组分；其侧链为正顺式十八碳-2-烯酰基；通过其铝络合物的质谱法确定了其纯度和分子结构。描述了通过薄层色谱法分离分枝杆菌素的铁络合物和铝络合物。地分枝杆菌、海分枝杆菌和耻垢分枝杆菌产生的分枝杆菌素彼此不同，且与分枝杆菌素P和T也不同。提出了分枝杆菌素及其衍生物的命名法。
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引用本文的文献
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Biochem J. 1965 Jan;94(1):160-5. doi: 10.1042/bj0940160.
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Biochem J. 1953 Nov;55(4):596-607. doi: 10.1042/bj0550596.
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J Gen Microbiol. 1966 Jun;43(3):329-43. doi: 10.1099/00221287-43-3-329.
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