Bakke A C, Bonner J
Biochemistry. 1979 Oct 16;18(21):4556-62. doi: 10.1021/bi00588a016.
Dictyostelium chromatin has been purified from nuclei in high yield by differential centrifugation and nuclease cleaving. Its chemical composition has been assayed, and its histones have been analyzed by gel electrophoresis, peptide fingerprints, amino acid composition, and ion-exchange chromatography. The mass ratios of DNA/RNA/histone/nonhistone are 1.0:0.18:0.98:1.02. There are four histones including one unusual histone, H7, which is the most abundant histone in the slime mold. The H4-like protein is the most conserved protein, while the other histones show both similarities and differences with mammalian histones.
通过差速离心和核酸酶切割，已从细胞核中高产率地纯化出盘基网柄菌染色质。已测定其化学组成，并通过凝胶电泳、肽指纹图谱、氨基酸组成和离子交换色谱法对其组蛋白进行了分析。DNA/RNA/组蛋白/非组蛋白的质量比为1.0:0.18:0.98:1.02。有四种组蛋白，包括一种不寻常的组蛋白H7，它是黏菌中最丰富的组蛋白。类H4蛋白是最保守的蛋白，而其他组蛋白与哺乳动物组蛋白既有相似之处，也有不同之处。
