Fenn W O, Marquis R E
J Gen Physiol. 1968 Nov;52(5):810-24. doi: 10.1085/jgp.52.5.810.
Growth of Streptococcus faecalis in a complex medium was inhibited by xenon, nitrous oxide, argon, and nitrogen at gas pressures of 41 atm or less. The order of inhibitory potency was: xenon and nitrous oxide > argon > nitrogen. Helium appeared to be impotent. Oxygen also inhibited streptococcal growth and it acted synergistically with narcotic gases. Growth was slowed somewhat by 41 atm hydrostatic pressure in the absence of narcotic gases, but the gas effects were greater than those due to pressure. In relation to the sensitivity of this bacterium to pressure, we found that the volume of cultures increased during growth in a volumeter or dilatometer, and that this dilatation was due mainly to glycolysis. A volume increase of 20.3 +/- 3.6 ml/mole of lactic acid produced was measured, and this value was close to one of 24 ml/mole lactic acid given for muscle glycolysis, and interestingly, close to the theoretic volume increase of activation calculated from the depression of growth rate by pressure.
在41个大气压及以下的气压条件下，粪肠球菌在复合培养基中的生长受到氙气、一氧化二氮、氩气和氮气的抑制。抑制效力顺序为：氙气和一氧化二氮＞氩气＞氮气。氦气似乎没有抑制作用。氧气也抑制链球菌生长，且与麻醉性气体协同作用。在没有麻醉性气体的情况下，41个大气压的静水压力会使生长稍有减缓，但气体的影响大于压力的影响。关于这种细菌对压力的敏感性，我们发现培养物在体积计或膨胀计中生长期间体积会增加，且这种膨胀主要归因于糖酵解。测得每产生1摩尔乳酸培养物体积增加20.3±3.6毫升，该值接近肌肉糖酵解中每摩尔乳酸24毫升的值，有趣的是，接近根据压力对生长速率的抑制作用计算出的理论活化体积增加量。
