Roitt I M, Torrigiani G, Doniach D
Immunology. 1968 Nov;15(5):681-96.
The binding of fluorescein-labelled thyroglobulin antibody from a Hashimoto patient to autologous thyroid could be completely inhibited by normal thyroglobulin showing that the autoantibody cannot detect any abnormality in the colloid protein from the diseased gland. Although a proportion of rabbit antisera to human thyroglobulin reacted with thyroxine, there was no evidence for such binding with human antibodies in the present studies. The heteroantibodies reacted almost identically with human and rhesus monkey thyroglobulin but much weaker cross-reactions were obtained with Hashimoto antibodies. This was related to the far smaller number of autoreactive determinants on the thyroglobulin molecule. Hashimoto sera giving a clear-line' with thyroglobulin in agar appear to react with fewer sites on the antigen than do precipitating sera, and from complexes of relatively small size in antibody excess. It was shown that sera giving horse-type' precipitin curves do not contain a mixture of avid non-precipitating antibodies with precipitating antibodies of lower affinity. It is postulated that thyroglobulin has two pairs of autoreactive determinants giving rise to two populations of antibodies. Clear-line sera contain almost exclusively one species or the other, horse-type' precipitating sera contain a preponderance of one or other species, and rabbit-type' sera contain comparable amounts of both types. A number of precipitation phenomena previously observed with Hashimoto sera can be explained on this hypothesis.
来自桥本氏病患者的荧光素标记甲状腺球蛋白抗体与自体甲状腺的结合可被正常甲状腺球蛋白完全抑制，这表明自身抗体无法检测到患病腺体胶体蛋白中的任何异常。尽管一部分针对人甲状腺球蛋白的兔抗血清与甲状腺素发生反应，但在本研究中没有证据表明人抗体存在这种结合。异种抗体与人及恒河猴甲状腺球蛋白的反应几乎相同，但与桥本氏抗体的交叉反应要弱得多。这与甲状腺球蛋白分子上自身反应性决定簇的数量少得多有关。在琼脂中与甲状腺球蛋白产生“清晰线条”的桥本氏血清似乎比沉淀血清与抗原上更少的位点发生反应，并且在抗体过量时形成相对较小尺寸的复合物。结果表明，产生“马型”沉淀素曲线的血清不包含高亲和力非沉淀抗体与低亲和力沉淀抗体的混合物。据推测，甲状腺球蛋白有两对自身反应性决定簇，产生两类抗体。清晰线条血清几乎只包含其中一种，“马型”沉淀血清中一种或另一种占优势，而“兔型”血清中两种类型的含量相当。基于这一假设，可以解释先前观察到的许多与桥本氏血清有关的沉淀现象。
