Ivannik B P, Golubeva R V, Proskuriakov S Ia, Riabchenko N I
Biokhimiia. 1979 Aug;44(8):1478-83.
The hormone-induced and post-irradiation changes in the molecular weight of a single-stranded DNA (SSDNA) in alkaline nuclear lysates and the activities of DNAses and pyknotic nuclei from rat thymocytes were studied. It was shown that 1 hr after injection of prednisolone (1 mg per 100 g of body weight) the molecular weight of SSDNA in the lymphoid organs is decreased with a subsequent increase by the 6th hour. The hormone-induced degradation of DNA is not accompanied by any marked increase in the activities of DNAses or by an appearance of pykotic nuclei in the thymocytes. The irradiation of the animals at a dose of 900 R leads to an irreversible decrease of the molecular weight of SSDNA in the lymphoid organs, to a steady increase of the DNAse activity and a sharp increase of the amount of pyknotic nuclei in the thymocytes. Studies on the mechanism of post-hormonal degradation of DNA in rat thymocytes in vitro demonstrated that prednisolone exerts its effects on the early and late stages of DNA degradation.
研究了激素诱导及照射后大鼠胸腺细胞碱性核裂解物中单链DNA（SSDNA）分子量的变化、DNA酶活性及核固缩核的情况。结果表明，注射泼尼松龙（每100克体重1毫克）1小时后，淋巴器官中SSDNA的分子量下降，随后在第6小时增加。激素诱导的DNA降解并未伴随DNA酶活性的显著增加或胸腺细胞中核固缩核的出现。以900伦琴的剂量照射动物会导致淋巴器官中SSDNA分子量不可逆下降、DNA酶活性持续增加以及胸腺细胞中核固缩核数量急剧增加。对大鼠胸腺细胞体外DNA激素后降解机制的研究表明，泼尼松龙对DNA降解的早期和晚期阶段均有影响。
