Kemp M B, Hegeman G D
J Bacteriol. 1968 Nov;96(5):1488-99. doi: 10.1128/jb.96.5.1488-1499.1968.
The regulation and genetic control of the beta-ketoadipate pathway in Pseudomonas aeruginosa were investigated. The pattern of enzyme induction is apparently the same as in P. putida. Mutants were obtained for all but 1 of the 11 structural genes; the proximity of these genes on the chromosome was examined by transduction of the mutants with phage F116. If a group of enzymes was induced by the same compounds, the corresponding genes were closely clustered. Surprisingly, some locispecifying enzymes not sharing a common inducer were also clustered. It is suggested that this latter finding may indicate a degree of chromosomal specialization.
对铜绿假单胞菌中β-酮己二酸途径的调控和基因控制进行了研究。酶诱导模式显然与恶臭假单胞菌中的相同。除了11个结构基因中的1个之外，其余基因的突变体均已获得；通过用噬菌体F116转导突变体来检查这些基因在染色体上的邻近性。如果一组酶由相同的化合物诱导，则相应的基因紧密聚集。令人惊讶的是，一些不共享共同诱导物的位点特异性酶也聚集在一起。有人认为，后一发现可能表明染色体存在一定程度的特化。
