铜绿假单胞菌异亮氨酸-缬氨酸突变体的生化与遗传分组
BIOCHEMICAL AND GENETIC GROUPING OF ISOLEUCINE-VALINE MUTANTS OF PSEUDOMONAS AERUGINOSA.
作者信息
PEARCE L E, LOUTIT J S
出版信息
J Bacteriol. 1965 Jan;89(1):58-63. doi: 10.1128/jb.89.1.58-63.1965.
Abstract
Pearce, L. E. (University of Otago, Dunedin, New Zealand), and J. S. Loutit. Biochemical and genetic grouping of isoleucine-valine mutants of Pseudomonas aeruginosa. J. Bacteriol. 89:58-63. 1965.-Isoleucine- and valine-requiring mutants of Pseudomonas aeruginosa, induced with ultraviolet and ethyl-methane-sulfonate, were isolated. The feeding properties and growth requirements of 56 of these mutants were examined, and evidence was found for at least four phenotypic groups, A, B, C, and D, controlling the biosynthesis of isoleucine and valine. Preliminary genetic mapping by use of bacteriophage-mediated transduction showed linkage between the mutant loci within groups A, B, and C. There was also evidence of linkage between the genes controlling groups A and B and some slight evidence of linkage between the other groups. There was insufficient evidence to suggest the arrangement of the genes.
摘要
皮尔斯，L.E.（新西兰达尼丁奥塔哥大学）和J.S.洛蒂特。铜绿假单胞菌异亮氨酸 - 缬氨酸突变体的生化和遗传分组。《细菌学杂志》89:58 - 63。1965年。——利用紫外线和甲磺酸乙酯诱导分离出了需要异亮氨酸和缬氨酸的铜绿假单胞菌突变体。检测了其中56个突变体的营养特性和生长需求，发现了至少四个表型组A、B、C和D控制异亮氨酸和缬氨酸的生物合成。通过噬菌体介导的转导进行的初步遗传图谱分析表明，A、B和C组内的突变位点之间存在连锁关系。也有证据表明控制A组和B组的基因之间存在连锁关系，其他组之间也有一些微弱的连锁证据。没有足够的证据来表明基因的排列方式。
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