Loh P C, Shatkin A J
J Virol. 1968 Nov;2(11):1353-9. doi: 10.1128/JVI.2.11.1353-1359.1968.
Polyacrylamide gel electrophoresis of the solubilized proteins from the three serotypes of reovirus revealed that each contained three major and four minor components. Subviral particles were prepared by brief treatment of complete virions with urea. Electron microscopy, density-gradient centrifugation, and chemical analyses of these particles indicated that their outer capsid structure had been selectively removed. They contained only two proteins, but their ribonucleic acid composition was similar to that of complete virions. The subviral particles were not infectious.
对呼肠孤病毒三种血清型的可溶性蛋白进行聚丙烯酰胺凝胶电泳分析发现,每种血清型均包含三个主要成分和四个次要成分。通过用尿素对完整病毒粒子进行短暂处理来制备亚病毒颗粒。对这些颗粒进行电子显微镜观察、密度梯度离心和化学分析表明,其外衣壳结构已被选择性去除。它们仅含有两种蛋白质,但其核糖核酸组成与完整病毒粒子相似。这些亚病毒颗粒没有感染性。