Lewandowski L J, Traynor B L
J Virol. 1972 Nov;10(5):1053-70. doi: 10.1128/JVI.10.5.1053-1070.1972.
Iodination of reovirus, cytoplasmic polyhedrosis virus (CPV), and wound tumor virus (WTV), and their respective subviral forms, followed by analysis of the labeled polypeptides by using polyacrylamide gel electrophoresis, has been used to compare the protein contents of these three diplornaviruses. This approach, when combined with electron microscopy and buoyant density determinations, appears capable of localizing individual polypeptides in some of the viral and subviral forms. CPV (p = 1.435 g/cm(3)) seems to resemble reovirus cores (p = 1.440 g/cm(3)) in both ultrastructure and polypeptide composition. CPV is composed of five polypeptides with molecular weights of about 151,000, 142,000, 130,000, 67,000, and 33,000. The polyhedral matrix, which in nature encapsulates the virions, is, in turn, composed mainly of two polypeptide species with molecular weights of about 30,000 and 20,000, and several minor proteins. The proteins of WTV consist mainly of four species of polypeptide with molecular weights of about 156,000, 122,000, 63,000, and 44,000, and several minor components. These molecular weight determinations are consistent with the hypothesis that, as has been suggested for reovirus, the viral proteins of CPV and WTV seem to be coded for by monocistronic mes senger RNA molecules transcribed from distinct segments of the double-stranded RNA viral genomes.
对呼肠孤病毒、细胞质多角体病毒（CPV）和伤口肿瘤病毒（WTV）及其各自的亚病毒形式进行碘化，然后通过聚丙烯酰胺凝胶电泳分析标记的多肽，已被用于比较这三种双链RNA病毒的蛋白质含量。当这种方法与电子显微镜和浮力密度测定相结合时，似乎能够在一些病毒和亚病毒形式中定位单个多肽。CPV（p = 1.435 g/cm³）在超微结构和多肽组成上似乎与呼肠孤病毒核心（p = 1.440 g/cm³）相似。CPV由五种分子量分别约为151,000、142,000、130,000、67,000和33,000的多肽组成。在自然界中包裹病毒粒子的多面体基质，反过来主要由两种分子量分别约为30,000和20,000的多肽物种以及几种次要蛋白质组成。WTV的蛋白质主要由四种分子量分别约为156,000、122,000、63,000和44,000的多肽物种以及几种次要成分组成。这些分子量测定结果与如下假设一致，即正如对呼肠孤病毒所提出的那样，CPV和WTV的病毒蛋白似乎由从双链RNA病毒基因组的不同片段转录而来的单顺反子信使RNA分子编码。
