Bozorgzadeh M H, Brenton A G, Wiebers J L, Beynon J H
Biomed Mass Spectrom. 1979 Aug;6(8):340-4. doi: 10.1002/bms.1200060806.
The technique of mass analysed ion kinetic energy spectroscopy has been applied to the structural determination of five different substituent sidechains from five steroids of similar ring anatomy. A mass spectrometer of reversed geometry, which permits ion selection according to its mass-to-charge ratio before the ions enter the electric sector, was used to isolate the ion corresponding to the mass of each particular sidechain. Subsequent mass analysed ion kinetic energy spectra of the selected ion demonstrated daughter ions whose compositions were compatible with the structures of the model compounds. This ability to determine the structure of a specific portion of a large molecule and to permit differentiation of minor structural features in a series of compounds, whose major framework is similar, adds a new dimension to the powerful mass analysed ion kinetic energy spectroscopy technique for structural analysis of complex biological molecules.
质量分析离子动能光谱技术已应用于结构相似的五种甾体化合物中五个不同取代基侧链的结构测定。使用一种逆向几何结构的质谱仪，该质谱仪允许离子在进入电场部分之前根据其质荷比进行选择，以分离出对应于每个特定侧链质量的离子。所选离子随后的质量分析离子动能光谱显示出其组成与模型化合物结构相符的子离子。这种确定大分子特定部分结构并区分一系列主要框架相似的化合物中微小结构特征的能力，为用于复杂生物分子结构分析的强大的质量分析离子动能光谱技术增添了新的维度。
