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[鹌鹑胚胎卵黄囊和后肢细胞移植后受辐照鸡骨髓中供体型造血集落的形成]

[Formation of hematopoietic colonies of the donor type in the bone marrow of irradiated chickens after transplantation of the cells of the yolk sac and hindlimb of the quail embryo].

作者信息

Latsis R V, Drize N I, Deriugina E I

出版信息

Biull Eksp Biol Med. 1979 Oct;88(10):432-4.

PMID:497381
Abstract

The ability of yolk sac and primary bone marrow cells of the quail to form hemopoietic colonies at 6 hours of incubation (i. e. before establishment of circulation) was studied in the bone marrow of 3-week sublethally irradiated chickens. The experiments were based on the possibility of differentiating between quail and chicken cells from the natural cell marker (Pheulgen-positive nucleolus). The number of hemopoietic colonies produced by cells transplanted from the primary bone marrow was three times greater than that consequent on transplantation of yolk sac cells. With the given dose of irradiation the bone marrow shows about 75% exogenous (quail) and 25% endogenous (chicken) hemopoietic colonies.

摘要

在3周龄经亚致死剂量照射的鸡的骨髓中,研究了鹌鹑的卵黄囊细胞和原代骨髓细胞在孵化6小时(即循环建立之前)形成造血集落的能力。实验基于利用天然细胞标志物(福尔根阳性核仁)区分鹌鹑和鸡细胞的可能性。从原代骨髓移植的细胞产生的造血集落数量比卵黄囊细胞移植后产生的数量大三倍。在给定的照射剂量下,骨髓中约75%为外源性(鹌鹑)造血集落,25%为内源性(鸡)造血集落。

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