Haas M, Yoshikawa H
J Virol. 1969 Feb;3(2):233-47. doi: 10.1128/JVI.3.2.233-247.1969.
PBSH, a defective phage of Bacillus subtilis strain 168, is described. Conditions are given for optimal induction of the prophage with mitomycin C. After a latent period of 90 min, cells were lysed and phage-like particles were released with a burst size of approximately 100 to 400 phage per bacterium. Since no known host supports phage replication after infection, burst size was determined by electron microscope count. Purification procedures and criteria for purity are described. The molecular weight of deoxyribonucleic acid (DNA) extracted from PBSH was estimated by length measurement and sedimentation. No circular DNA molecules were found by either technique. PBSH DNA molecules are linear, double-stranded, and of homogeneous molecular weight, about 12 x 10(6) daltons. There is no evidence for single-strand breaks. The majority of PBSH DNA molecules show a sedimentation behavior dependent on ionic strength. It is inferred that most of the DNA molecules are less hydrodynamically rigid than native DNA having a similar average base composition and molecular weight. Possible reasons for the sedimentation behavior are discussed.
本文描述了枯草芽孢杆菌168菌株的缺陷噬菌体PBSH。给出了用丝裂霉素C最佳诱导原噬菌体的条件。潜伏期90分钟后，细胞裂解，释放出噬菌体样颗粒，每个细菌的爆发量约为100至400个噬菌体。由于感染后没有已知宿主支持噬菌体复制，爆发量通过电子显微镜计数确定。描述了纯化程序和纯度标准。通过长度测量和沉降估计从PBSH中提取的脱氧核糖核酸（DNA）的分子量。两种技术均未发现环状DNA分子。PBSH DNA分子是线性的、双链的，分子量均匀，约为12×10⁶道尔顿。没有单链断裂的证据。大多数PBSH DNA分子的沉降行为取决于离子强度。据推测，大多数DNA分子的流体动力学刚性低于具有相似平均碱基组成和分子量的天然DNA。讨论了沉降行为的可能原因。
