Yehle C O, Brenner D J
J Virol. 1970 Jan;5(1):39-44. doi: 10.1128/JVI.5.1.39-44.1970.
Radiolabeled phage SPO2 fragments were tested for the abiity to form interspecies deoxyribonucleic acid (DNA) duplexes with DNA from Bacillus subtilis and from phages beta3 and beta22. No reassociation, above control values, occurred between the DNA of phage SPO2 and that of its host or either of the virulent B. subtilis phages. It appears that extensive nucleotide sequence similarities between portions of host and phage DNA species are not necessary for the formation of the lysogenic state. The thermal elution profile of reassociated SPO2 DNA exhibited a normal distribution, indicating no heterogeneity in base composition. This differs from temperate enterophages whose DNA molecules contain regions of differing base composition.
对放射性标记的噬菌体SPO2片段进行了测试，以检测其与枯草芽孢杆菌以及噬菌体β3和β22的DNA形成种间脱氧核糖核酸（DNA）双链体的能力。噬菌体SPO2的DNA与其宿主或任何一种毒性枯草芽孢杆菌噬菌体的DNA之间，均未出现高于对照值的重新结合现象。看来，宿主和噬菌体DNA物种部分之间广泛的核苷酸序列相似性对于溶原状态的形成并非必要。重新结合的SPO2 DNA的热洗脱图谱呈正态分布，表明碱基组成没有异质性。这与温和性肠道噬菌体不同，后者的DNA分子含有碱基组成不同的区域。
