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通过乳胶凝集法测定动物对肺炎支原体的抗体反应。

Antibody response of animals to Mycoplasma pneumoniae measured by latex agglutination.

作者信息

Kende M

出版信息

Appl Microbiol. 1969 Feb;17(2):275-9. doi: 10.1128/am.17.2.275-279.1969.

DOI:10.1128/am.17.2.275-279.1969
PMID:4975657
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC377664/
Abstract

The standardization, application, and usefulness of latex agglutination for Mycoplasma pneumoniae antibody titration were investigated and compared with tetrazolium reduction inhibition and complement fixation. The sera of guinea pigs and monkeys reacted in a specific fashion, whereas rabbit serum required pretreatment to eliminate its nonspecific agglutinin. Human serum given such pretreatment still contained a nonspecific agglutinin. The latex agglutination procedure compared favorably with complement fixation and metabolic inhibition tests for evaluating vaccine antigenicity.

摘要

对用于肺炎支原体抗体滴定的乳胶凝集试验的标准化、应用及实用性进行了研究，并与四氮唑还原抑制试验和补体结合试验进行了比较。豚鼠和猴的血清呈现出特异性反应，而兔血清需要进行预处理以消除其非特异性凝集素。经过这种预处理的人血清仍含有非特异性凝集素。在评估疫苗抗原性方面，乳胶凝集试验与补体结合试验及代谢抑制试验相比具有优势。

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本文引用的文献

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Application of a microtechnique to viral serological investigations.一种微技术在病毒血清学研究中的应用。
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Proc Soc Exp Biol Med. 1961 Jan;106:168-70. doi: 10.3181/00379727-106-26273.
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Use of latex particles in serology of tuberculosis.乳胶颗粒在结核病血清学中的应用。
Am Rev Respir Dis. 1967 Apr;95(4):638-42. doi: 10.1164/arrd.1967.95.4.638.
6
Mycoplasma-latex agglutination reaction.支原体乳胶凝集反应。
J Bacteriol. 1966 Oct;92(4):1196-205. doi: 10.1128/jb.92.4.1196-1205.1966.
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Antimetabolic antibodies to Mycoplasma pneumoniae measured by tetrazolium reduction inhibition.
Proc Soc Exp Biol Med. 1966 Jul;122(3):786-90. doi: 10.3181/00379727-122-31252.
8
The effect of kaolin on immunoglobulins: an improved technique to remove the nonspecific serum inhibitor of reovirus hemagglutination.高岭土对免疫球蛋白的影响：一种去除呼肠孤病毒血凝反应非特异性血清抑制剂的改良技术。
J Immunol. 1967 Jun;98(6):1136-42.

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