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产黑色素拟杆菌口腔菌株脂多糖的血清学特性

Serological properties of lipopolysaccharide from oral stains of Bacteroides melaninogenicus.

作者信息

Hofstad T

出版信息

J Bacteriol. 1969 Mar;97(3):1078-82. doi: 10.1128/jb.97.3.1078-1082.1969.

Abstract

Lipopolysaccharide (LPS) extracted with phenol-water from four oral strains of Bacteroides melaninogenicus was found to be serologically active in precipitation and complement fixation tests and sensitized sheep erythrocytes to agglutination. Except for the capacity to inhibit indirect hemagglutination, the serological activity was destroyed by oxidation with periodate. The isolated LPS was antigenic in rabbits, giving rise to low- and high-molecular-weight antibodies. Cross-reactivity experiments revealed the presence in LPS of both type-specific and group-reactive antigenic determinants.

摘要

用酚水法从四株产黑色素拟杆菌口腔菌株中提取的脂多糖,在沉淀试验和补体结合试验中具有血清学活性,且能使绵羊红细胞致敏发生凝集。除了抑制间接血凝的能力外,其血清学活性可被高碘酸盐氧化破坏。分离出的脂多糖在兔体内具有抗原性,可产生低分子量和高分子量抗体。交叉反应实验表明脂多糖中存在型特异性和群反应性抗原决定簇。

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本文引用的文献

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Fractionation of human-serum proteins by gel filtration.通过凝胶过滤法分离人血清蛋白。
Biochim Biophys Acta. 1962 Oct 8;63:402-10. doi: 10.1016/0006-3002(62)90104-x.
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Serologically specific lipopolysaccharides from oral veillonella.
Arch Oral Biol. 1963 Jan-Feb;8:31-6. doi: 10.1016/0003-9969(63)90089-x.

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