Yasui H, Yasutake N, Ohwaki M
Infect Immun. 1979 Apr;24(1):39-46. doi: 10.1128/iai.24.1.39-46.1979.
Three different strains of Bacteroides were isolated from feces and cecal contents of mice. The immunogenicity of the strains was determined by measuring the serum agglutinin titers after intraperitoneal antigen injection. There were marked differences in quantity and quality of produced antibodies among the three strains. One strain (2-2) induced low antibody titers in both the primary and secondary responses, and a significant 2-mercaptoethanol (2-ME)-resistant antibody production occurred. Another strain (Y) induced low antibody titers in the primary response and high titers in the secondary response, but 2-ME-resistant antibody production did not occur. The third strain (2-4) induced very high antibody titers in both the responses, and a large amount of 2-ME-resistant antibody production occurred. Further, heat-ethanol-treated strain Y induced only immunoglobulin M antibody, but periodate-treated strain Y induced no antibody. Heat-ethanol- or periodate-treated strain 2-4 induced immunoglobulin M or G antibody, respectively. These observations suggest that the surface antigens of the two strains are distinctly different: the antigen of strain Y would be mostly O-antigen, whereas those of strain 2-4 would be O-antigen and protein moieties.
从小鼠粪便和盲肠内容物中分离出三种不同的拟杆菌菌株。通过腹腔内注射抗原后测量血清凝集素效价来确定这些菌株的免疫原性。这三种菌株产生的抗体在数量和质量上存在显著差异。一种菌株（2-2）在初次和二次应答中均诱导低抗体效价，并且出现了显著的对2-巯基乙醇（2-ME）抗性的抗体产生。另一种菌株（Y）在初次应答中诱导低抗体效价，在二次应答中诱导高效价，但未出现对2-ME抗性的抗体产生。第三种菌株（2-4）在两种应答中均诱导非常高的抗体效价，并且出现了大量对2-ME抗性的抗体产生。此外，经热乙醇处理的菌株Y仅诱导免疫球蛋白M抗体，但经高碘酸盐处理的菌株Y未诱导抗体产生。经热乙醇或高碘酸盐处理的菌株2-4分别诱导免疫球蛋白M或G抗体产生。这些观察结果表明，这两种菌株的表面抗原明显不同：菌株Y的抗原主要为O抗原，而菌株2-4的抗原为O抗原和蛋白质部分。
