枯草芽孢杆菌中谷氨酰胺合成酶合成的调控
Regulation of synthesis of glutamine synthase in Bacillus subtilis.
作者信息
Rebello J L, Strauss N
出版信息
J Bacteriol. 1969 May;98(2):683-8. doi: 10.1128/jb.98.2.683-688.1969.
Abstract
A study of the regulation of the synthesis of the enzyme glutamine synthase in Bacillus subtilis was initiated. An assay, based on the measurement of glutamo-hydroxamate, was used to characterize the enzyme in crude preparations and in toluene-treated cells. Determinations were made of the Michaelis constants for adenosine triphosphate, hydroxylamine, and glutamate (9 x 10(-3), 4 x 10(-3), and 2.2 x 10(-2)m, respectively), the pH optimum (7.6 to 7.7), and the stability. The differential rate of synthesis was determined under various growth conditions. The enzyme was found to be relatively insensitive to regulation. Partial repression was caused by glutamine, arginine, asparagine, and glutamate, or by carbon limitation in a chemostat. Derepression was caused by exhaustion of externally added amino acids or by nitrogen limitation in a chemostat.
摘要
对枯草芽孢杆菌中谷氨酰胺合成酶合成调控的研究已经展开。基于谷氨羟肟酸测量的一种测定法，被用于表征粗制品和经甲苯处理的细胞中的该酶。测定了三磷酸腺苷、羟胺和谷氨酸的米氏常数（分别为9×10⁻³、4×10⁻³和2.2×10⁻²m）、最适pH（7.6至7.7）以及稳定性。在各种生长条件下测定了合成的差异速率。发现该酶对调控相对不敏感。谷氨酰胺、精氨酸、天冬酰胺和谷氨酸，或恒化器中的碳限制会导致部分阻遏。外部添加的氨基酸耗尽或恒化器中的氮限制会导致去阻遏。
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