Gardner A L, Aronson A I
J Bacteriol. 1984 Jun;158(3):967-71. doi: 10.1128/jb.158.3.967-971.1984.
The structural gene for glutamine synthetase (glnA) in Bacillus subtilis ( glnAB ) cloned in the lambda vector phage Charon 4A was used to transduce a lysogenic glutamine auxotrophic Escherichia coli strain to prototrophy. The defective E. coli gene ( glnAE ) was still present in the transductant since it could be transduced. In addition, curing of the prototroph resulted in the restoration of glutamine auxotrophy. Proteins in crude extracts of the transductant were examined by a "Western blotting" procedure for the presence of B. subtilis or E. coli glutamine synthetase antigen; only the former was detected. Growth of the strain in media without glutamine was not curtailed even when the bacteriophage lambda pL and pRM promoters were hyperrepressed . The specific activities and patterns of derepression of glutamine synthetase in the transductant were similar to those of B. subtilis, with no evidence for adenylylation. The information necessary for regulation of glnAB must be closely linked to the gene and appears to function in E. coli.
克隆于λ载体噬菌体Charon 4A的枯草芽孢杆菌谷氨酰胺合成酶（glnA）的结构基因（glnAB），被用于将溶原性谷氨酰胺营养缺陷型大肠杆菌菌株转导为原养型。转导子中仍存在缺陷的大肠杆菌基因（glnAE），因为它可以被转导。此外，原养型的治愈导致谷氨酰胺营养缺陷型的恢复。通过“蛋白质免疫印迹”程序检测转导子粗提物中的蛋白质，以确定是否存在枯草芽孢杆菌或大肠杆菌谷氨酰胺合成酶抗原；仅检测到前者。即使噬菌体λ pL和pRM启动子被高度抑制，该菌株在无谷氨酰胺的培养基中生长也未受到抑制。转导子中谷氨酰胺合成酶的比活性和去阻遏模式与枯草芽孢杆菌相似，没有腺苷酸化的证据。glnAB调控所需的信息必须与该基因紧密相连，并且似乎在大肠杆菌中起作用。
