阿尔茨海默病中染色质构象的改变。

Altered chromatin conformation in Alzheimer's disease.

作者信息

Crapper D R, Quittkat S, de Boni U

出版信息

Brain. 1979 Sep;102(3):483-95. doi: 10.1093/brain/102.3.483.

DOI:10.1093/brain/102.3.483

Abstract

Chromatin samples were prepared from forty human brains. Chromatin was separated into a heavy heterochromatin fraction and two euchromatin fractions: intermediate euchromatin and light euchromatin. Employing a bacterial RNA polymerase as probe, only the euchromatin fractions were capable of RNA synthesis. In Control human brains, in brains of patients with dialysis dementia and in brains of elderly individuals without or with dementia of a type other than Alzheimer's disease, the euchromatin fractions accounted for about 75 per cent of the total DNA. In contrast, in brains of patients with advanced senile dementia or presenile dementia of the Alzheimer type, a wide range of euchromatin content was encountered with an average value of 55 per cent. Heterochromatization occurred in both neuron and glia enriched fractions suggesting that a major alteration in protein metabolism occurs in Alzheimer's disease.

摘要

从四十个人类大脑中制备染色质样本。染色质被分离成一个重异染色质部分和两个常染色质部分：中间常染色质和轻常染色质。以细菌RNA聚合酶作为探针，只有常染色质部分能够进行RNA合成。在对照人类大脑、透析性痴呆患者的大脑以及无痴呆或患有除阿尔茨海默病以外其他类型痴呆的老年人的大脑中，常染色质部分约占总DNA的75%。相比之下，在患有晚期老年性痴呆或早老性阿尔茨海默型痴呆患者的大脑中，常染色质含量范围很广，平均值为55%。在富含神经元和神经胶质的部分均发生了异染色质化，这表明阿尔茨海默病中蛋白质代谢发生了重大改变。