McLachlan D R, Lewis P N, Lukiw W J, Sima A, Bergeron C, De Boni U
Ann Neurol. 1984 Apr;15(4):329-34. doi: 10.1002/ana.410150405.
Nuclei extracted from neocortex of patients with Alzheimer's disease and treated with micrococcal nuclease release a population of dinucleosomes that contain an increase in the linker histones H1o and H1oo . Five other degenerative brain diseases that clinically resemble Alzheimer's disease do not result in these changes, although Pick's disease is associated with an increase in H1 on dinucleosomes. Histones from nuclei of patients with Alzheimer's disease are also more resistant to salt-induced release from chromatin than are those from age-matched control subjects. These results support the hypothesis that an alteration in chromatin structure is a marker for Alzheimer's disease.
从阿尔茨海默病患者的新皮质中提取细胞核，并用微球菌核酸酶处理后，会释放出一群二核小体，这些二核小体中的连接组蛋白H1o和H1oo含量增加。另外五种临床上与阿尔茨海默病相似的退行性脑病并不会导致这些变化，尽管匹克病与二核小体上H1的增加有关。与年龄匹配的对照受试者相比，阿尔茨海默病患者细胞核中的组蛋白对盐诱导的从染色质上释放也更具抗性。这些结果支持了染色质结构改变是阿尔茨海默病标志物这一假说。
