嗜盐细菌不同菌株的脱氧核糖核酸特性分析
Characterization of the deoxyribonucleic acid of various strains of halophilic bacteria.
作者信息
Moore R L, McCarthy B J
出版信息
J Bacteriol. 1969 Jul;99(1):248-54. doi: 10.1128/jb.99.1.248-254.1969.
Abstract
Bacteria classified as extreme halophiles, in the genera Halobacterium and Halococcus, contain deoxyribonucleic acid (DNA) which displays two components in a CsCl equilibrium density gradient. The base composition of the major DNA component ranges from 66 to 68% guanine plus cytosine (GC), whereas that of the satellite DNA comprising some 11 to 36% of the total, is between 57 and 60% GC. Purification of the bacterial cells in a CsCl density gradient and other more conventional strain purification procedures both indicated that the presence of the satellite DNA component is not a result of mixed cultures.
摘要
归类为嗜极嗜盐菌的细菌，如盐杆菌属和盐球菌属，其脱氧核糖核酸（DNA）在氯化铯平衡密度梯度中呈现出两个组分。主要DNA组分的碱基组成中鸟嘌呤加胞嘧啶（GC）含量在66%至68%之间，而占总量约11%至36%的卫星DNA的GC含量在57%至60%之间。在氯化铯密度梯度中对细菌细胞进行纯化以及其他更传统的菌株纯化程序均表明，卫星DNA组分的存在并非混合培养的结果。
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