通过氯化铯中浮力密度测量推导硫酸盐还原菌脱氧核糖核酸的碱基组成
Base composition of deoxyribonucleic acid of sulfate-reducing bacteria deduced from buoyant density measurements in cesium chloride.
作者信息
Saunders G F, Campbell L L, Postgate J R
出版信息
J Bacteriol. 1964 May;87(5):1073-8. doi: 10.1128/jb.87.5.1073-1078.1964.
Saunders, Grady F. (University of Illinois, Urbana), L. Leon Campbell, and John R. Postgate. Base composition of deoxyribonucleic acid of sulfate-reducing bacteria deduced from buoyant density measurements in cesium chloride. J. Bacteriol. 87:1073-1078. 1964.-The base composition of the deoxyribonucleic acid (DNA) of sulfate-reducing bacteria was calculated from buoyant density measurements in CsCl. The sporulating sulfate-reducing bacteria fell into two groups: Desulfovibrio orientis with a DNA base composition of 42% guanine plus cytosine (G + C), and Clostridium nigrificans with a DNA base composition of 45% G + C. The mesophilic relative of C. nigrificans had a DNA base composition of 46% G + C. Thirty strains of nonsporulating sulfate-reducing bacteria called D. desulfuricans were studied. They fell into three groups as judged by DNA base composition: group I (11 strains), 60 to 62% G + C; group II (13 strains), 54 to 56% G + C; and group III (6 strains), 46 to 47% G + C. These data underline the need for a taxonomic revision of this group of microorganisms.
桑德斯，格雷迪·F.（伊利诺伊大学厄巴纳分校）、L. 利昂·坎贝尔和约翰·R. 波斯盖特。通过氯化铯中的浮力密度测量推断硫酸盐还原菌脱氧核糖核酸的碱基组成。《细菌学杂志》87:1073 - 1078。1964年。——通过氯化铯中的浮力密度测量计算了硫酸盐还原菌脱氧核糖核酸（DNA）的碱基组成。产芽孢的硫酸盐还原菌分为两组：东方脱硫弧菌，其DNA碱基组成为42%鸟嘌呤加胞嘧啶（G + C），以及黑化梭菌，其DNA碱基组成为45% G + C。黑化梭菌的嗜温亲缘种DNA碱基组成为46% G + C。研究了30株称为脱硫脱硫弧菌的不产芽孢硫酸盐还原菌。根据DNA碱基组成判断，它们分为三组：第一组（11株），G + C为60%至62%；第二组（13株），G + C为54%至56%；第三组（6株），G + C为46%至47%。这些数据强调了对这组微生物进行分类修订的必要性。
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