乳酸杆菌属的脱氧核糖核酸碱基组成
Deoxyribonucleic acid base composition of the genus Lactobacillus.
作者信息
Gasser F, Mandel M
出版信息
J Bacteriol. 1968 Sep;96(3):580-8. doi: 10.1128/jb.96.3.580-588.1968.
Abstract
Deoxyribonucleic acids of 45 strains of Lactobacillus and 5 strains of Bifidobacterium which had been analyzed for base composition by chromatographic means were examined at equilibrium in a CsCl density gradient. Regression analysis showed that there have been systematic errors involved in the estimation of guanine plus cytosine (GC) content by the chemical method, and that the relation between buoyant density and base composition is indeed linear and best fitted by the equation GC = 10.309 (rho-1.662), which compares well in slope with the equation of Schildkraut, Marmur, and Doty. With the improved data obtained in this study, the specific groupings of the species of both genera were reevaluated.
摘要
对45株乳酸杆菌和5株双歧杆菌的脱氧核糖核酸进行了研究，这些菌株此前已通过色谱法分析了碱基组成，并在CsCl密度梯度中达到平衡时进行了检测。回归分析表明，化学方法估算鸟嘌呤加胞嘧啶（GC）含量时存在系统误差，且浮力密度与碱基组成之间的关系确实呈线性，最佳拟合方程为GC = 10.309（ρ - 1.662），该方程的斜率与Schildkraut、Marmur和Doty的方程相当。根据本研究获得的改进数据，对这两个属的物种的具体分组进行了重新评估。
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