库蠓微孢子虫：体外分离

Nosema cuiculi: in vitro isolation.

作者信息

Shadduck J A

出版信息

Science. 1969 Oct 24;166(3904):516-7. doi: 10.1126/science.166.3904.516.

DOI:10.1126/science.166.3904.516

Abstract

A mammalian microsporidian was propagated for the first time in vitro with rabbit choroid plexus monolayer cultures. Tentative classification as Nosema cuniculi (Levaditi, 1923) was established by morphologic and tinctorial characteristics and by extrusion of sintgle polar filaments with attached sporoplasin from living spores. The method permits propagation of large numbers of organisms in putre cultutre.

摘要

一种哺乳动物微孢子虫首次在兔脉络丛单层培养物中进行体外培养。通过形态学和染色特性以及从活孢子中挤出带有附着孢子质的单极丝，初步将其分类为兔脑炎微孢子虫（Levaditi，1923年）。该方法允许在纯培养物中大量繁殖这种生物体。