Waller T
Lab Anim. 1975 Jan;9(1):61-8. doi: 10.1258/002367775780994835.
Growth patterns of Nosema cuniculi (Encephalitozoon cuniculi) in cell cultures of bovine kidney, canine kidney, feline lung, and rabbit kidney were studied. All cell cultures used were easy to manage and the last 3 are commercially-available established cell lines. The dog kidney cells were the most suitable for large-scale production of Nosema. When grown in plastic flasks with a bottom area of 75 cm2, the weekly yield from Nosema-infected canine kidney cells during the 10th to 17th week after inoculation was between 4-1 times 10-7 and 9-9 times 10-7 spores per flask. An equilibrium was obtained between the Nosema infection and the kidney cells during this time. A simple method for estimating the number of harvested spores is also described.
研究了兔脑炎微孢子虫（脑胞内原虫）在牛肾、犬肾、猫肺和兔肾细胞培养物中的生长模式。所有使用的细胞培养物都易于管理，后三种是市售的已建立细胞系。犬肾细胞最适合大规模生产兔脑炎微孢子虫。当在底部面积为75平方厘米的塑料培养瓶中培养时，接种后第10至17周，感染兔脑炎微孢子虫的犬肾细胞每周产量为每瓶4×10⁻⁷至9×10⁻⁷个孢子。在此期间，兔脑炎微孢子虫感染与肾细胞之间达到了平衡。还描述了一种估计收获孢子数量的简单方法。
