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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Shetty A S, Miller G W

Biochem J. 1969 Sep;114(2):331-7. doi: 10.1042/bj1140331.

delta-Aminolaevulate dehydratase (EC 4.2.1.24) was purified 80-fold from tobacco leaves and its properties were studied. 2. The enzyme had optimum pH7.4 in potassium phosphate buffer, K(m)6.25x10(-4)m at 37 degrees and pH7.4, optimum temperature 45 degrees and an activation energy of 11100 cal./mole. 3. The enzyme lost activity when prepared in the absence of cysteine, and this activity was only partly restored by the later addition of thiols. Reagents for thiol groups inactivated the enzyme. 4. Mg(2+) was essential for activity, and EDTA and Fe(2+) were inhibitory; Mn(2+) was an activator or an inhibitor depending on the concentration.

从烟草叶片中纯化出δ-氨基乙酰丙酸脱水酶（EC 4.2.1.24），并对其性质进行了研究。2. 该酶在磷酸钾缓冲液中的最适pH为7.4，在37℃和pH7.4时的米氏常数K(m)为6.25×10⁻⁴m，最适温度为45℃，活化能为11100卡/摩尔。3. 在无半胱氨酸的情况下制备该酶时会失去活性，后期添加硫醇只能部分恢复其活性。硫醇基团试剂会使该酶失活。4. Mg²⁺对活性至关重要，EDTA和Fe²⁺具有抑制作用；Mn²⁺根据浓度不同既是激活剂又是抑制剂。

Shetty A S, Miller G W

Biochem J. 1969 Sep;114(2):331-7. doi: 10.1042/bj1140331.

delta-Aminolaevulate dehydratase (EC 4.2.1.24) was purified 80-fold from tobacco leaves and its properties were studied. 2. The enzyme had optimum pH7.4 in potassium phosphate buffer, K(m)6.25x10(-4)m at 37 degrees and pH7.4, optimum temperature 45 degrees and an activation energy of 11100 cal./mole. 3. The enzyme lost activity when prepared in the absence of cysteine, and this activity was only partly restored by the later addition of thiols. Reagents for thiol groups inactivated the enzyme. 4. Mg(2+) was essential for activity, and EDTA and Fe(2+) were inhibitory; Mn(2+) was an activator or an inhibitor depending on the concentration.

从烟草叶片中纯化出δ-氨基乙酰丙酸脱水酶（EC 4.2.1.24），并对其性质进行了研究。2. 该酶在磷酸钾缓冲液中的最适pH为7.4，在37℃和pH7.4时的米氏常数K(m)为6.25×10⁻⁴m，最适温度为45℃，活化能为11100卡/摩尔。3. 在无半胱氨酸的情况下制备该酶时会失去活性，后期添加硫醇只能部分恢复其活性。硫醇基团试剂会使该酶失活。4. Mg²⁺对活性至关重要，EDTA和Fe²⁺具有抑制作用；Mn²⁺根据浓度不同既是激活剂又是抑制剂。