Lundberg B
Chem Phys Lipids. 1979 Jun-Jul;24(3):257-63. doi: 10.1016/0009-3084(79)90031-8.
The solubility of the three steroid hormones, progesterone, testosterone, and estradiol-17 beta in water and phosphatidylcholine vesicles was measured after shaking and ultrasonication. All three steroids have low water solubility, which increases considerably at sonication for testosterone and estradiol-17 beta. The phosphatidylcholine vesicles have a very small solubilising capacity for the steroids; about 20 mumol/mol. This increases at sonication for estradiol-17 beta and decreases for testosterone. The capacity for progesterone is almost unaltered. The incorporation of cholesterol in the vesicles decreased the solubilisation capacity for testosterone and estradiol-17 beta but increased that for progesterone of shaked preparations. For the sonicated systems the cholesterol decreased the solubilising capacity for estradiol-17 beta but increased that for testosterone. The solubilisation experiments indicate that the steroid hormones are solubilised in the hydrocarbon part of the phosphatidylcholine bilayer and also 13CNMR results support this conclusion.
在振荡和超声处理后，测定了三种甾体激素（孕酮、睾酮和雌二醇-17β）在水和磷脂酰胆碱囊泡中的溶解度。所有这三种甾体激素在水中的溶解度都很低，对于睾酮和雌二醇-17β，超声处理后其溶解度显著增加。磷脂酰胆碱囊泡对甾体激素的增溶能力非常小；约为20 μmol/mol。对于雌二醇-17β，超声处理后该能力增加，而对于睾酮则降低。孕酮的能力几乎未改变。在囊泡中加入胆固醇降低了对睾酮和雌二醇-17β的增溶能力，但增加了振荡制剂中孕酮的增溶能力。对于超声处理的体系，胆固醇降低了对雌二醇-17β的增溶能力，但增加了对睾酮的增溶能力。增溶实验表明，甾体激素溶解在磷脂酰胆碱双层的烃类部分，并且13C NMR结果也支持这一结论。
