Tojo T, Friou G J, Spiegelberg H L
Clin Exp Immunol. 1970 Jan;6(1):145-51.
Fourteen sera containing antinuclear antibodies were studied to determine whether the immunoglobulin G subclass of these antibodies correlated with the relative complement fixing activity of the antibodies or the presence or absence of lupus nephritis. Sera studied included eight with high complement fixing activity, all from patients with active lupus nephritis and six with low complement fixing activity, three from patients with lupus without nephritis and three from patients with antinuclear antibodies induced by procainamide. Purified antinuclear antibodies reactive with desoxyribonucleoprotein and desoxyribonuclease were prepared by elution, and subclass typing was carried out by radial immunoassay with specific antisera. In general, subclasses represented were similar in frequency to the occurrence of the subclasses in normal immunoglobulin G. There was no striking predominance or absence of any single subclass which might correlate with presence of nephritis, or differing complement fixing activity of sera. There was a trend to a predominance of subclasses high in complement fixing activity in antinuclear antibodies from lupus patients with active nephritis and subclasses low in complement fixing activity from those without nephritis.
对14份含有抗核抗体的血清进行了研究，以确定这些抗体的免疫球蛋白G亚类是否与抗体的相对补体固定活性或狼疮性肾炎的有无相关。所研究的血清包括8份具有高补体固定活性的血清，均来自活动性狼疮性肾炎患者；以及6份具有低补体固定活性的血清，其中3份来自无肾炎的狼疮患者，3份来自由普鲁卡因胺诱导产生抗核抗体的患者。通过洗脱制备与脱氧核糖核蛋白和脱氧核糖核酸酶反应的纯化抗核抗体，并使用特异性抗血清通过放射免疫测定法进行亚类分型。一般来说，所代表的亚类在频率上与正常免疫球蛋白G中亚类的出现情况相似。没有任何一个单一亚类明显占优势或缺失，这可能与肾炎的存在或血清不同的补体固定活性相关。在有活动性肾炎的狼疮患者的抗核抗体中，有补体固定活性高的亚类占优势的趋势，而在无肾炎的患者中，有补体固定活性低的亚类占优势的趋势。
