Eisenberg R A, Dyer K, Craven S Y, Fuller C R, Yount W J
J Clin Invest. 1985 Apr;75(4):1270-7. doi: 10.1172/JCI111826.
Anti-Sm antibodies are highly specific markers for the diagnosis of systemic lupus erythematosus (SLE). This specificity suggests that the immunoregulation of these autoantibodies would reflect fundamental immune abnormalities in this disorder. As a clue to this immunoregulation, we have investigated the isotype distribution of anti-Sm antibodies by enzyme-linked immunosorbent assays. We have found that the anti-Sm response is markedly restricted to the IgG1 heavy chain isotype. On the other hand, the light chain distribution reflects that in normal serum, while isoelectric focusing analysis fails to show an oligoclonal pattern. The related specificity, anti-ribonucleoprotein, is also restricted to IgG1, while the SLE-specific antibody anti-double-stranded DNA is mostly IgG1 with a lesser contribution by IgG3. These results suggest that antinuclear antibodies that are strongly associated with SLE are produced by a T cell-dependent response, probably driven by antigen. The immunoregulation of the response to several autoantigens may be quite similar.
抗Sm抗体是诊断系统性红斑狼疮（SLE）的高度特异性标志物。这种特异性表明，这些自身抗体的免疫调节反映了该疾病中基本的免疫异常。作为这种免疫调节的线索，我们通过酶联免疫吸附测定法研究了抗Sm抗体的同种型分布。我们发现抗Sm反应明显局限于IgG1重链同种型。另一方面，轻链分布反映了正常血清中的情况，而异电聚焦分析未能显示寡克隆模式。相关的特异性抗核糖核蛋白也局限于IgG1，而SLE特异性抗体抗双链DNA主要是IgG1，IgG3的贡献较小。这些结果表明，与SLE密切相关的抗核抗体是由T细胞依赖性反应产生的，可能由抗原驱动。对几种自身抗原反应的免疫调节可能非常相似。
