来自感染淋巴细胞性脉络丛脑膜炎病毒的BHK - 21细胞培养物的补体结合抗原。
Complement-fixing antigen from BHK-21 cell cultures infected with lymphocytic choriomeningitis virus.
作者信息
Brown W J, Kirk B E
出版信息
Appl Microbiol. 1969 Sep;18(3):496-9. doi: 10.1128/am.18.3.496-499.1969.
Abstract
Infection of BHK-21 cells with lymphocytic choriomeningitis (LCM) virus resulted in the production of significant titers of complement-fixing (CF) antigen. The antigen was spontaneously released from the cells, but the highest titer of 1:16 was recovered by disruption of the infected cells by freeze-thawing in tryptose phosphate broth. The antigen could be partially separated from infectious virus by centrifugation. Furthermore, it was possible to detect LCM virus infection of cell cultures by the production of the CF antigen, but this method proved less sensitive than titration by intracerebral inoculation of mice. The CF antigen from cell cultures was at least as sensitive and specific as the reference antigen prepared from infected guinea pig spleen.
摘要
用淋巴细胞性脉络丛脑膜炎（LCM）病毒感染BHK - 21细胞会产生高滴度的补体结合（CF）抗原。该抗原会自发地从细胞中释放出来，但通过在胰蛋白胨磷酸盐肉汤中冻融破坏感染细胞，可获得最高滴度为1:16的抗原。通过离心可将该抗原与感染性病毒部分分离。此外，通过检测CF抗原的产生能够检测细胞培养物中的LCM病毒感染，但该方法被证明不如通过小鼠脑内接种滴定法敏感。来自细胞培养物的CF抗原至少与从感染豚鼠脾脏制备的参考抗原一样敏感和特异。
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