“Percoll”密度梯度中的人红细胞组分
Human erythrocyte fraction in "Percoll" density gradients.
作者信息
Rennie C M, Thompson S, Parker A C, Maddy A
出版信息
Clin Chim Acta. 1979 Oct 15;98(1-2):119-25. doi: 10.1016/0009-8981(79)90172-4.
PMID:498523
Abstract
A new rapid method for the age fractionation of human erythrocytes by centrifugation through a "Percoll" density gradient is described. The fractionation is demonstrated by density-related changes in the volume, haemoglobin concentration, pyruvate kinase and acetylcholine esterase activities and potassium contents of the erythrocytes, and the distribution of reticulocytes on the gradients.
摘要
本文描述了一种通过“Percoll”密度梯度离心对人红细胞进行年龄分级的新快速方法。通过红细胞体积、血红蛋白浓度、丙酮酸激酶和乙酰胆碱酯酶活性以及钾含量与密度相关的变化，以及网织红细胞在梯度上的分布来证明分级情况。
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