Williams D M
Clin Chim Acta. 1979 Nov 15;99(1):23-9. doi: 10.1016/0009-8981(79)90135-9.
Using atomic absorption spectrophotometry, we determined levels of iron, copper and zinc in serum or plasma samples collected in acid-washed containers and in commercially available evacuated tubes including one model equipped with rubber stoppers prepared by a process which reduces zinc contamination of the rubber. Serum or plasma from all evacuated tubes yielded iron and copper values which were comparable to those from serum obtained in acid-washed glass. Zinc values were higher from all evacuated tubes than from acid-washed glassware. Values obtained from samples collected in the specially-prepared tubes were 10% higher than values obtained from samples collected in acid-washed glassware. Thus, the specially-prepared evacuated tubes may only be useful in large-scale clinical screening studies. Traditional blood collection methods for zinc determination should be used when accuracy is required.
我们使用原子吸收分光光度法，测定了在酸洗容器以及市售真空采血管（包括一种配备通过减少橡胶锌污染工艺制备的橡胶塞的型号）中采集的血清或血浆样本中的铁、铜和锌含量。所有真空采血管中的血清或血浆产生的铁和铜值与酸洗玻璃管中获得的血清中的值相当。所有真空采血管中的锌值均高于酸洗玻璃器皿中的锌值。在特制采血管中采集的样本所获得的值比在酸洗玻璃器皿中采集的样本所获得的值高10%。因此，特制的真空采血管可能仅在大规模临床筛查研究中有用。当需要准确性时，应使用传统的锌测定采血方法。
