Guerrasio A, Saglio G, Mazza U, Pich P, Camaschella C, Ricco G, Gianazza E, Righetti P G
Clin Chim Acta. 1979 Nov 15;99(1):7-11. doi: 10.1016/0009-8981(79)90133-5.
Electrophoresis in cellulose acetate in the presence of 3% Nonidet P-40 can resolve two neutral genetic variants, A gamma and G gamma human fetal globin chains. The ratio of these two chains, determined by densitometry of the electrophoretic strips, is in excellent agreement with the Gly-Ala ratio obtained by chemical analysis of the cyanogen bromide fragment gamma CB3. It is suggested that the detergent binds preferentially to the hydrophobic amino acid segment 133-141 in the A gamma chain, thus masking either a Lys or an Arg residue at the two extremes.
在含有3%聚氧乙烯辛基苯基醚（Nonidet P - 40）的醋酸纤维素上进行电泳，可以分离出两种中性遗传变体，即人胎儿血红蛋白的Aγ链和Gγ链。通过对电泳条带进行光密度测定得出的这两条链的比例，与通过对溴化氰片段γCB3进行化学分析得到的甘氨酸 - 丙氨酸比例高度一致。有人提出，这种去污剂优先结合到Aγ链中133 - 141位的疏水氨基酸片段上，从而掩盖了两端的一个赖氨酸或精氨酸残基。
