Barton J, Smith M B, Cauchi M N
Am J Hematol. 1978;5(4):341-5. doi: 10.1002/ajh.2830050409.
A technique involving the quantitative estimation of globin chains using cellulose acetate electrophoresis is described, and applied to the analysis of fetal globin chain production. When cord blood was analyzed, there was poor correlation between the globin chain ratios namely beta/alpha, gamma/alpha and beta/gamma ratios with either age or weight of newborn babies between 36-40 weeks gestation: best correlations were obtained for the beta/gamma ratio against weight (P less than 0.05). However, there was highly significant correlation between the various globin chain ratios and either age or weight of the newborn infant up to 12 weeks of age (P less than 0.001). This technique allows visual demonstration and quantitation of abnormal haemoglobins in a single procedure.
本文描述了一种利用醋酸纤维素电泳对珠蛋白链进行定量估计的技术，并将其应用于胎儿珠蛋白链产生的分析。对脐血进行分析时，在妊娠36 - 40周的新生儿中，珠蛋白链比率（即β/α、γ/α和β/γ比率）与年龄或体重之间的相关性较差：β/γ比率与体重的相关性最佳（P小于0.05）。然而，在出生后12周龄以内的新生儿中，各种珠蛋白链比率与年龄或体重之间存在高度显著的相关性（P小于0.001）。该技术可在单一程序中直观显示并定量分析异常血红蛋白。
