Chai N C, Lark K G
J Bacteriol. 1970 Mar;101(3):1005-13. doi: 10.1128/jb.101.3.1005-1013.1970.
After starvation for deoxyribosides, the deoxyribonucleic acid (DNA) of Lactobacillus acidophilus is restricted to a localized region of the cell. (3)H-uracil is first incorporated into such a restricted region but subsequently is found throughout the cell. This spread occurs despite the absence of protein synthesis and a major reduction in the rate of ribonucleic acid (RNA) synthesis. However, blocking RNA synthesis with actinomycin D restricts incorporation to a localized region of the cell. It is concluded that uracil is first incorporated into RNA in the bacterial nucleus from which it subsequently spreads through the cell. Actinomycin D could prevent this spread by preventing the completion of RNA molecules, which therefore do not dissociate from the DNA template.
在缺乏脱氧核糖核苷的饥饿状态下，嗜酸乳杆菌的脱氧核糖核酸（DNA）局限于细胞的一个局部区域。（3）H-尿嘧啶首先掺入这样一个受限区域，但随后在整个细胞中都能发现。尽管缺乏蛋白质合成且核糖核酸（RNA）合成速率大幅降低，但这种扩散仍会发生。然而，用放线菌素D阻断RNA合成会将掺入限制在细胞的一个局部区域。得出的结论是，尿嘧啶首先在细菌细胞核中掺入RNA，随后从细胞核扩散到整个细胞。放线菌素D可以通过阻止RNA分子的完成来防止这种扩散，因此RNA分子不会从DNA模板上解离。
