Soska J, Koukalová B, Reich J
Biochim Biophys Acta. 1976 Sep 20;447(1):45-52. doi: 10.1016/0005-2787(76)90094-0.
In Lactobacillus acidophilus, the DNA synthesis in cells incubated in the absence of essential amino acids reaches levels corresponding to the initiation of further replication cycles than just to completing the cycles already running. This "relaxation of DNA synthesis" is stimulated by the presence of inhibitors of protein synthesis and by the presence of deoxyadenylate. These enhancements of DNA replication are cancelled by actinomycin D. In the presence of inhibitors of protein synthesis, the relaxtion of DNA synthesis is further stimulated by free amino acids. The effect of free amino acids, unlike in the former cases, is not inhibited by actinomycin D. It proposed that the chromosome replication in Lactobacillus acidophilus is regulated similarly as in some plasmids, i.e. independently of the synthesis of protein, depending on the synthesis of RNA and, in addition, on the presence of free amino acids.
在嗜酸乳杆菌中，在缺乏必需氨基酸的情况下培养的细胞中的DNA合成达到的水平对应于进一步复制周期的起始，而不仅仅是完成已经在进行的周期。这种“DNA合成的松弛”受到蛋白质合成抑制剂的存在和脱氧腺苷酸的存在的刺激。这些DNA复制的增强被放线菌素D消除。在蛋白质合成抑制剂存在的情况下，游离氨基酸进一步刺激DNA合成的松弛。与前几种情况不同，游离氨基酸的作用不受放线菌素D的抑制。有人提出，嗜酸乳杆菌中的染色体复制与某些质粒中的调节方式类似，即独立于蛋白质合成，取决于RNA的合成，此外还取决于游离氨基酸的存在。
