Hughes-Jones N C, Gardner B
Immunology. 1970 Mar;18(3):347-51.
Estimates have been made of the value of the equilibrium constant and of the heterogeneity index of the reaction between anti-D and Rh positive red cells, using anti-D immunoglobulin preparations made from pools of donor plasma. The values of the equilibrium constant all fell within the range 1×10 to 10×10 1/mole, and in eighteen of the twenty-five samples the values fell within the narrow range of 2×10 to 4×10 1/mole. Estimates of the value of the heterogeneity index varied from 0.5 to 0.8. Calculations made using these values of the equilibrium constant indicate that if 200 μg of anti-D from each preparation were injected into an Rh-negative recipient with approximately 3 ml of Rr cells in the circulation, between 8 and 28 per cent (average 17 per cent) of the D antigen sites on the red cells would be bound by anti-D if equilibrium conditions were reached.
利用从供体血浆池中制备的抗-D免疫球蛋白制剂，对抗-D与Rh阳性红细胞之间反应的平衡常数和异质性指数的值进行了估算。平衡常数的值均落在1×10至10×10 1/摩尔的范围内，在25个样本中的18个样本中，该值落在2×10至4×10 1/摩尔的狭窄范围内。异质性指数的值在0.5至0.8之间变化。使用这些平衡常数的值进行的计算表明，如果将每种制剂中的200μg抗-D注射到循环中有大约3ml Rr细胞的Rh阴性受者体内，若达到平衡条件，红细胞上8%至28%（平均17%）的D抗原位点将被抗-D结合。
