Rutberg L, Armentrout R W
J Virol. 1972 Oct;10(4):658-60. doi: 10.1128/JVI.10.4.658-660.1972.
Increased deoxyribonucleic acid (DNA) polymerase activity is found in soluble extracts from a polymerase I-negative mutant of Bacillus subtilis after infection with temperate phage SPO2, or after induction of SPO2 prophage in lysogenic derivatives of this mutant. No increased enzyme activity is found after SPO2 infection in the presence of chloramphenicol. Infection of the polymerase-negative mutant with the DNA-negative sus mutant SPO2 L244 gives no increased enzyme activity, whereas infection with DNA-negative sus mutant SPO2 J385 gives enzyme activities comparable to those found in wild-type infected cells. These findings suggest that SPO2 determines a DNA polymerase activity essential for synthesis of phage DNA.
在被温和噬菌体SPO2感染后，或在该突变体的溶源衍生物中诱导SPO2原噬菌体后，在枯草芽孢杆菌聚合酶I阴性突变体的可溶性提取物中发现脱氧核糖核酸（DNA）聚合酶活性增加。在氯霉素存在的情况下，SPO2感染后未发现酶活性增加。用DNA阴性的sus突变体SPO2 L244感染聚合酶阴性突变体不会增加酶活性，而用DNA阴性的sus突变体SPO2 J385感染则会产生与野生型感染细胞中相当的酶活性。这些发现表明，SPO2决定了噬菌体DNA合成所必需的DNA聚合酶活性。
