蛋白质-烃类相互作用。各种蛋白质与纯癸烷的相互作用。
Protein-hydrocarbon interactions. Interactions of various proteins with pure decane.
作者信息
Cecil R, Louis C F
出版信息
Biochem J. 1970 Mar;117(1):139-45. doi: 10.1042/bj1170139.
Abstract
- Solutions of a number of proteins were subjected to gentle agitation in the presence of small quantities of decane. 2. Some of the protein was lost from solution and adsorbed on the surface of the emulsion formed; at the same time some of the decane was bound to the protein remaining in solution. 3. The two processes were found to be related and a mechanism is proposed to explain the relationship. 4. With lysozyme and ribonuclease, the protein in the aqueous phase of an emulsion exhibited normal enzymic activity, whereas the fraction adsorbed on the interface was much less active but recovered activity on desorption.
摘要
- 若干种蛋白质溶液在少量癸烷存在下进行轻柔搅拌。2. 部分蛋白质从溶液中损失并吸附在形成的乳液表面；与此同时，部分癸烷与留在溶液中的蛋白质结合。3. 发现这两个过程相关，并提出一种机制来解释这种关系。4. 对于溶菌酶和核糖核酸酶，乳液水相中的蛋白质表现出正常的酶活性，而吸附在界面上的部分活性则低得多，但解吸后恢复活性。
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