Rezwan K, Studart A R, Vörös J, Gauckler L J
Nonmetallic Inorganic Materials, Department Materials, ETH Zurich, Switzerland.
J Phys Chem B. 2005 Aug 4;109(30):14469-74. doi: 10.1021/jp050528w.
The amounts of negatively charged bovine serum albumin and positively charged lysozyme adsorbed on alumina, silica, titania, and zirconia particles (diameters 73 to 271 nm) in aqueous suspensions are measured. The adsorbed proteins change the zeta potentials and the isoelectric points (IEP) of the oxide particles. The added to adsorbed protein ratios at pH 7.5 are compared with the protein treated particle zeta potentials. It is found that the amounts of adsorbed proteins on the alumina, silica, and titania (but not on the zirconia) particle surfaces are highly correlated with the zeta potential. For the slightly less hydrophilic zirconia particles high amounts of protein adsorption are observed even under repulsive electrostatic conditions. One reason could be that the hydrophobic effect plays a more important role for zirconia than electrostatic interaction.
测量了在水悬浮液中吸附在氧化铝、二氧化硅、二氧化钛和氧化锆颗粒（直径73至271纳米）上的带负电荷的牛血清白蛋白和带正电荷的溶菌酶的量。吸附的蛋白质会改变氧化物颗粒的zeta电位和等电点（IEP）。将pH值为7.5时添加的蛋白质与吸附的蛋白质的比例与经蛋白质处理的颗粒zeta电位进行比较。结果发现，氧化铝、二氧化硅和二氧化钛（但不包括氧化锆）颗粒表面上吸附的蛋白质的量与zeta电位高度相关。对于亲水性稍差的氧化锆颗粒，即使在排斥性静电条件下也观察到大量的蛋白质吸附。一个原因可能是疏水作用对氧化锆比静电相互作用起更重要的作用。
