Buhr M M, Carlson J C, Thompson J E
Endocrinology. 1979 Dec;105(6):1330-5. doi: 10.1210/endo-105-6-1330.
Wide angle x-ray diffraction has been used to examine the phase behavior of microsomal membranes from regressing corpora lutea of prepubertal pseudopregnant rats. During periods of optimal progesterone secretion, all of the membrane lipid was in the liquid-crystalline phase at physiological temperature and, therefore, was fluid. However, mixtures of liquid-crystalline and gel phase lipid were observed under identical conditions in microsomal membrane preparations from animals undergoing spontaneous or prostaglandin F2 alpha-induced regression. This was accompanied by a parallel rise in the lipid phase transition temperature. In addition, the proportion of lipid in the gel phase increased with time after prostaglandin F2 alpha treatment. These results indicate that the mechanism of corpus luteum regression may involve phase changes in the phospholipid bilayer of cellular membranes. The resulting presence of gel phase lipid in the membrane matrices could contribute to the loss of tissue function.
广角X射线衍射已被用于研究青春期前假孕大鼠退化黄体微粒体膜的相行为。在孕酮分泌最佳的时期，所有膜脂在生理温度下均处于液晶相，因此是流体状态。然而，在经历自发或前列腺素F2α诱导退化的动物的微粒体制剂中，在相同条件下观察到了液晶相和凝胶相脂质的混合物。这伴随着脂质相变温度的平行升高。此外，前列腺素F2α处理后，凝胶相脂质的比例随时间增加。这些结果表明，黄体退化的机制可能涉及细胞膜磷脂双层的相变。膜基质中凝胶相脂质的出现可能导致组织功能丧失。
