Carlson J C, Buhr M M, Gruber M Y, Thompson J E
Endocrinology. 1981 Jun;108(6):2124-8. doi: 10.1210/endo-108-6-2124.
Wide angle x-ray diffraction has revealed that during corpus luteum regression there is a liquid-crystalline to gel phase transition in the phospholipid molecules of the cellular membranes. In the present study we have examined the lipid composition of these membranes and looked for evidence of membrane protein involvement in this change. Lipid analysis of smooth microsomal membranes prepared from rat corpora lutea revealed no significant change in the cholesterol to phospholipid ratio or in the ratio of unsaturated to saturated fatty acids with advancing luteolysis. In addition, there was no clear trend for these changes in the relative proportions of the major fatty acids. Liposomes were prepared from smooth microsomal fractions of regressing rat corpora lutea, and examination of these lipid vesicles by x-ray diffraction revealed that the temperature of the liquid-crystalline to gel phase transition was much lower (approximately 25-30 C) than that for the corresponding microsomes. These observations are consistent with the view that membrane proteins contribute to the ordering of lipid that results in a mixture of liquid-crystalline and gel phases in membranes from regressed corpora lutea.
广角X射线衍射显示，在黄体退化过程中，细胞膜磷脂分子存在从液晶相向凝胶相的转变。在本研究中，我们检测了这些膜的脂质组成，并寻找膜蛋白参与这一变化的证据。对从大鼠黄体制备的平滑微粒体膜进行脂质分析发现，随着黄体溶解的进展，胆固醇与磷脂的比例或不饱和脂肪酸与饱和脂肪酸的比例均无显著变化。此外，主要脂肪酸的相对比例也没有明显的变化趋势。从退化大鼠黄体的平滑微粒体组分制备脂质体，通过X射线衍射对这些脂质囊泡进行检测，结果显示液晶相向凝胶相转变的温度比相应微粒体的温度低得多（约25 - 30℃）。这些观察结果与以下观点一致，即膜蛋白有助于脂质的有序排列，从而导致退化黄体膜中出现液晶相和凝胶相的混合。
